How Peptide Batch Testing Works

A batch (or lot) is a quantity of material produced in a single, continuous manufacturing run under the same conditions. Everything in the batch is assumed to be homogeneous, which is what allows a sample taken from it to represent the whole. Each batch receives a unique reference that follows it from synthesis to the label on the vial, and every document generated along the way is filed against that reference.

After synthesis, purification and lyophilisation, a sample is drawn from the batch for analysis. Where material has been filled into vials, the sample is a sealed vial from the run rather than loose powder, so that the test reflects what a customer receives. The sample is logged with the batch reference and dispatched to the testing laboratory with a chain-of-custody record.

The first question is whether the material is the compound it is supposed to be. Mass spectrometry measures the molecular weight of the sample and compares it with the theoretical mass calculated from the sequence. For a peptide such as BPC-157 the expected monoisotopic mass is known precisely, and a match within the instrument's tolerance confirms identity. Where there is any doubt, tandem MS fragments the peptide and reads the sequence directly.

The sample is then run by reversed-phase HPLC and the area of the main peak is expressed as a percentage of all peaks. The method parameters are recorded. For a batch to be released, purity must meet the specification, which for our catalogue is a minimum of 98% with most batches above 99%. The chromatogram is attached to the certificate so that the result can be inspected rather than taken on trust.

Results are reviewed against the specification. If identity is confirmed and purity meets the limit, a certificate of analysis is issued for the batch. If either fails, the batch is quarantined and not released. Only after release are vials labelled with the final batch reference and made available for dispatch.

Our testing is performed by Janoshik Analytical, an independent laboratory, and each certificate is published on the laboratory's own verification site. That matters: a certificate hosted only by the seller can be edited; one hosted by the lab cannot.

Take the batch reference from your vial label, search it on our lab reports page, and open the certificate. Confirm that the compound name and strength match the label, that the method is stated, that the purity result is above specification, and that mass spectrometry confirms identity. If anything does not match, contact us with the batch reference and we will investigate.